Goat anti-NMDA receptor 1 / GRIN1 Antibody

Pep ELISA, WB

Goat anti-NMDA receptor 1 / GRIN1 Antibody
Principle Name: NMDA receptor 1 / GRIN1
Official Symbol: GRIN1
All Name and Symbols: GRIN1; NMDA receptor 1; glutamate receptor, ionotropic, N-methyl D-aspartate 1 ; NMDA1; NMDAR1; NR1 ; N-methyl-D-aspartate receptor channel, subunit zeta-1; glutamate [NMDA] receptor subunit zeta 1
Accession Number(s): NP_000823.4; NP_067544.1; NP_015566.1
Peptide Sequence: TQERVNNSNKKE, is from internal region
Human Gene ID(s): 2902
Non-Human GeneID(s): 14810 (mouse); 24408 (rat);
Important Comments: This antibody is expected to recognise all three reported isoforms (NP_000823.4; NP_067544.1; NP_015566.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.Peptide ELISA: antibody detection limit dilution 1 to 1000.
Western Blot: Approx 100kDa band observed in Human Brain (Amylgada) and Rat Brain lysates (calculated MW of 101kDa according to Human NP_067544.1 and Rat NP_001257534.1. Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. Preliminary test
Reference(s): Rajji T, Chapman D, Eichenbaum H, Greene R. The role of CA3 hippocampal NMDA receptors in paired associate learning. J Neurosci. 2006 Jan 18; 26(3):908-15. .PMID: 16421310