Goat anti-Tensin 1 / TNS1 Antibody

Goat anti-Tensin 1 / TNS1 Antibody
Principle Name: Tensin 1 / TNS1
Official Symbol: TNS1
All Name and Symbols: TNS1; tensin 1; TNS; tensin; MGC88584; PRO0929; FLJ10923; DKFZP434G162; MSTP091; DKFZp586K0617; MST091; MST122; MST127; MSTP122; MSTP127; MXRA6; Matrix-remodelling-associated protein 6; matrix-remodelling associated 6; tensin
Accession Number(s): NP_072174.3
Peptide Sequence: VSKVMLNAGQKR, is from C Terminus
Human Gene ID(s): 7145
Non-Human GeneID(s): 21961 (mouse); 301509 (rat);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, IHC
Tested Species:
Peptide ELISA: antibody detection limit dilution 1 to 2000.
Western Blot: Preliminary experiments in Human Heart, Kidney and Skeletal Muscle lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results bee
Immunohistochemistry: In paraffin embedded Human Kidney shows weak staining of the basement membranes of convoluted tubules. Recommended concentration, 4-6ug/ml.
Reference(s): Chen H, Ishii A, Wong WK, Chen LB, Lo SH. Molecular characterization of human tensin. Biochem J. 2000 Oct 15; 351 Pt 2:403-11..PMID: 11023826