Goat anti-Quaking / QKI Antibody

Pep ELISA, WB

Goat anti-Quaking / QKI Antibody
Principle Name: Quaking / QKI
Official Symbol: QKI
All Name and Symbols: QKI; quaking homolog, KH domain RNA binding; DKFZp586I0923; Hqk; QK; QK3 ; RNA binding protein HQK; homolog of mouse quaking QKI (KH domain RNA binding protein); quaking homolog, KH domain RNA binding
Accession Number(s): NP_006766.1; NP_996735.1; NP_996736.1; NP_996737.1
Peptide Sequence: ETKEKPKPTPDY, is from N Terminus
Human Gene ID(s): 9444
Non-Human GeneID(s): 19317 (mouse);
Important Comments: This antibody is expected to recognise all reported isoforms (NP_006766.1; NP_996735.1; NP_996736.1; NP_996737.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Approx 38kDa band observed in mouse brain lysates (calculated MW of 35.8kDa according to NP_068681.1). Recommended concentration: 0.3-1ug/ml.
Reference(s): Wu JI, Reed RB, Grabowski PJ, Artzt K. Function of quaking in myelination: regulation of alternative splicing. Proc Natl Acad Sci U S A. 2002 Apr 2; 99(7):4233-8. Epub 2002 Mar 26. .PMID: 11917126