Goat anti-PAK1 Antibody

Goat anti-PAK1 Antibody
Principle Name: PAK1
Official Symbol: PAK1
All Name and Symbols: PAK1; p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) ; MGC130000; MGC130001; PAKalpha ; p21-activated kinase 1; p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
Accession Number(s): NP_002567.3
Peptide Sequence: NTEKQKKKPKMSDE, is from internal region
Human Gene ID(s): 5058
Non-Human GeneID(s): 18479 (mouse); 29431 (rat);
Important Comments: No cross-reactivity exptected with PAK3
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WBPeptide ELISA: antibody detection limit dilution 1 to 1000.
Western Blot: Approx 65kDa band observed in Human Brain (Cerebral Cortex and Hippocampus) lysates (calculated MW of 60.6kDa according to NP_002567.3). Recommended concentration: 0.5-1.5ug/ml.
Reference(s): Zhao L, Ma QL, Calon F, Harris-White ME, Yang F, Lim GP, Morihara T, Ubeda OJ, Ambegaokar S, Hansen JE, Weisbart RH, Teter B, Frautschy SA, Cole GM. Role of p21-activated kinase pathway defects in the cognitive deficits of Alzheimer disease. Nat Neurosci. 2006 Feb; 9(2):234-42. Epub 2006 Jan 15. .PMID: 16415866