Goat anti-Lipin 3 Antibody

Pep ELISA, IHC

Goat anti-Lipin 3 Antibody
Principle Name: Lipin 3
Official Symbol: LPIN3
All Name and Symbols: LPIN3; lipin 3 ; RP4-620E11.3; LIPN3L; SMP2; dJ450M14.2; dJ450M14.3; dJ620E11.2 ; lipin 3-like
Accession Number(s): NP_075047.1
Peptide Sequence: KPKQKEDAVATD, is from internal region
Human Gene ID(s): 64900
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Preliminary experiments gave bands at approx 80kDa and 60kDa in Human Liver lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated s
Immunohistochemistry: In paraffin embedded Human Thyroid Gland shows staining of cytoplasm and cell membrane of epithelial cells surrounding the follicles. Recommended concentration, 2-3ug/ml.
Reference(s): Santos-Rosa H, Leung J, Grimsey N, Peak-Chew S, Siniossoglou S. The yeast lipin Smp2 couples phospholipid biosynthesis to nuclear membrane growth. EMBO J. 2005 Jun 1; 24(11):1931-41. Epub 2005 May 5. .PMID: 15889145