Goat anti-Nur77 / TR3 (isoform a) Antibody

Goat anti-Nur77 / TR3 (isoform a) Antibody
Principle Name: Nur77 / TR3 (isoform a)
Official Symbol: NR4A1
All Name and Symbols: NR4A1; NUR77; nuclear receptor subfamily 4, group A, member 1 ; GFRP1; HMR; MGC9485; N10; NAK-1; NGFIB; NP10; TR3 ; TR3 orphan receptor; early response protein NAK1; growth factor-inducible nuclear protein N10; hormone receptor; orphan nuclear receptor HM
Accession Number(s): NP_002126.2; NP_775180.1
Peptide Sequence: GRLPSKPKQPPDAS, is from internal region
Human Gene ID(s): 3164
Non-Human GeneID(s): 15370 (mouse); 79240 (rat);
Important Comments: This antibody is expected to recognise isoform a (NP_002126.2 and NP_775180.1) only.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 60kDa band observed in Human Thyroid gland lysates (calculated MW of 64.5kDa according to NP_002126.2 and NP_775180.1). Recommended concentration: 1-3ug/ml.
Immunohistochemistry: Paraffin embedded Human Breast. Recommended concentration: 5ug/ml.
Reference(s): Kiss I, Oskolas H, Toth R, Bouillet P, Toth K, Fulop A, Scholtz B, Ledent C, Fesus L, Szondy Z. Adenosine A(2A )receptor-mediated cell death of mouse thymocytes involves adenylate cyclase and Bim and is negatively regulated by Nur77. Eur J Immunol. 2006 Jun; 36(6):1559-71. .PMID: 16673448