Goat anti-MBD2 (isoform 1) Antibody

Pep ELISA, WB, IHC

Goat anti-MBD2 (isoform 1) Antibody
Principle Name: MBD2 (isoform 1)
Official Symbol: MBD2
All Name and Symbols: MBD2; methyl-CpG binding domain protein 2 ; DKFZp586O0821; DMTase; NY-CO-41
Accession Number(s): NP_003918.1
Peptide Sequence: RNDPLNQNKGKPDLN, is from internal region
Human Gene ID(s): 8932
Non-Human GeneID(s): 17191 (mouse);
Important Comments: This antibody is expected to resognize isoform 1 (NP_003918.1) only.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Approx 48-50kDa band observed in lysates of cell line Jurkat (calculated MW of 43.3kDa according to NP_003918.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Tan et al, Mol Cell Bio
Immunohistochemistry: In paraffin embedded Human Colon shows nuclear stianing of enterocytes. Recommended concentration, 2-4ug/ml.
Reference(s): Auriol E, Billard LM, Magdinier F, Dante R. Specific binding of the methyl binding domain protein 2 at the BRCA1-NBR2 locus. Nucleic Acids Res. 2005 Jul 28; 33(13):4243-54. Print 2005. .PMID: 16052033