Goat anti-TCF2 / VHNF1 Antibody

Goat anti-TCF2 / VHNF1 Antibody
Principle Name: TCF2 / VHNF1
Official Symbol: HNF1B
All Name and Symbols: TCF2; VHNF1; transcription factor 2, hepatic LF-B3 variant hepatic nuclear factor; FJHN; HNF1B; HNF1beta; HNF2; LFB3; MODY5; transcription factor 2
Accession Number(s): NP_000449.1; NP_001159395.1
Peptide Sequence: QAYDRQKNPSKEER, is from internal region
Human Gene ID(s): 6928
Non-Human GeneID(s): 21410 (mouse); 25640 (rat);
Important Comments: This antibody is expected to recognize both reported isoforms (NP_000449.1; NP_001159395.1). This product may cross-react with HNF1A.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, ICC
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Approx 60kDa band observed in Human Liver lysates (calculated MW of 61.3kDa according to NP_000449.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.
An additional band of unknown identity was also consistently o
Reference(s): Terasawa K, Toyota M, Sagae S, Ogi K, Suzuki H, Sonoda T, Akino K, Maruyama R, Nishikawa N, Imai K, Shinomura Y, Saito T, Tokino T. Epigenetic inactivation of TCF2 in ovarian cancer and various cancer cell lines. Br J Cancer. 2006 Mar 27; 94(6):914-21. .PMID: 16479257