Goat anti-PDE4D3 Antibody

Pep ELISA, WB

Goat anti-PDE4D3 Antibody
Principle Name: PDE4D3
Official Symbol: PDE4D
All Name and Symbols: PDE4D3; phosphodiesterase 4D3;
Accession Number(s): NP_006194.2
Peptide Sequence: HVNNFPFRRHS, is from N Terminus
Human Gene ID(s): 5144
Non-Human GeneID(s): 24627 (mouse);
Important Comments: This antibody is expected to recognize isoform 3 (NP_006194.2) only.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 75kDa band observed in Rat Brain lysates (calculated MW of 76.5kDa according to human NP_006194.2). Recommended concentration: 0.01-0.03ug/ml.
Reference(s): Houslay, M.D., Baillie, G.S. and Maurice, D.H. cAMP-Specific phosphodiesterase-4 enzymes in the cardiovascular system: a
molecular toolbox for generating compartmentalized cAMP signaling. Circ Res. 2007 Apr 13; 100(7):950-66. Review.PMID: 17431197