Goat anti-ATGL / Desnutrin Antibody

Pep ELISA, WB, IHC

Goat anti-ATGL / Desnutrin Antibody
Principle Name: ATGL / Desnutrin
Official Symbol: PNPLA2
All Name and Symbols: ATGL; DESNUTRIN; PNPLA2; patatin-like phospholipase domain containing 2; 1110001C14Rik; DKFZp667M109; FP17548; PEDF-R; TTS-2.2; TTS2;
adipose triglyceride lipase; pigment epithelium-derived factor; transport-secretion protein 2.2; triglyceride hydrolase
Accession Number(s): NP_065109.1
Peptide Sequence: NIIEVSKEARKR, is from internal region
Human Gene ID(s): 57104
Non-Human GeneID(s): 66853 (mouse); 361676 (rat);
Important Comments: Pease note this antibody was designed using the Mouse sequence NP_080078.1, which differs by 2 amino acids from the Human sequence NP_065109.1.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 60kDa band observed in Human Adipose lysates (calculated MW of 55.3kDa according to NP_065109.1). Recommended concentration: 0.5-2ug/ml.
Immunohistochemistry: In paraffin embedded Human Heart shows intense staining of distinct spots in the myocardial fibres in transverse section. Recommended concentration: 2-3ug/ml.
Reference(s): Schoenborn V, Heid IM, Vollmert C, Lingenhel A, Adams TD, Hopkins PN, Illig T, Zimmermann R, Zechner R, Hunt SC, Kronenberg F. The ATGL gene is associated with free fatty acids, triglycerides, and type 2 diabetes. Diabetes. 2006 May; 55(5):1270-5. .PMID: 16644682