Goat anti-Actin-like 7B (mouse) Antibody

Goat anti-Actin-like 7B (mouse) Antibody
Principle Name: Actin-like 7B (mouse)
Official Symbol: Actl7b
All Name and Symbols: actin-like 7B; ACTL7B; MGC151192, MGC151194, Tact1; actin-like 7-beta; t-actin 1
Accession Number(s): NP_079547.1
Peptide Sequence: DELHVDYELPDGK, is from internal region
Human Gene ID(s):
Non-Human GeneID(s): 11471 (mouse); 313183 (rat);
Important Comments: This antibody is not expected to cross-react with Actin-like 7A.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Rat
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 45kDa band observed in Rat Testis lysates (calculated MW of 45.5kDa according to Rat NP_001020588.1 and 45.6kDa according to Mouse NP_079547.1). Recommended concentration: 0.5-1.5ug/ml.
An additional band of unknown identity was als
Reference(s): Chadwick BP, Mull J, Helbling LA, Gill S, Leyne M, Robbins CM, Pinkett HW, Makalowska I, Maayan C, Blumenfeld A, Axelrod FB, Brownstein M, Gusella JF, Slaugenhaupt SA. Cloning, mapping, and expression of two novel actin genes, actin-like-7A (ACTL7A) and actin-like-7B (ACTL7B), from the familial dysautonomia candidate region on 9q31. Genomics. 1999 Jun 15; 58(3):302-9. .PMID: 10373328