Goat anti-APPL1 Antibody

Goat anti-APPL1 Antibody
Principle Name: APPL1
Official Symbol: APPL1
All Name and Symbols: APPL1; DIP13alpha ; adaptor protein containing pH domain, PTB domain and leucine zipper motif; signaling adaptor protein DIP13alpha
Accession Number(s): NP_036228.1
Peptide Sequence: NRYSRLSKKRENDK, is from internal region
Human Gene ID(s): 26060
Non-Human GeneID(s): 72993 (mouse);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species:
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Approx 80kDa band observed in Rat Aortic Smooth Skeletal Muscle Cells (lane 1), intensity increased after infection with Human APPL1 adenovirus for 48h (lane 2). Calculated MW of 79.7kDa according to NP_036228.1.
The blot was co-labeled wit
Reference(s): Mao X, Kikani CK, Riojas RA, Langlais P, Wang L, Ramos FJ, Fang Q, Christ-Roberts CY, Hong JY, Kim RY, Liu F, Dong LQ. APPL1 binds to adiponectin receptors and mediates adiponectin signalling and function. Nat Cell Biol. 2006 May; 8(5):516-23. Epub 2006 Apr 16. Erratum in: Nat Cell Biol. 2006 Jun; 8(6):642..PMID: 16622416