Goat anti-PIAS2 Antibody

Pep ELISA, IHC

Goat anti-PIAS2 Antibody
Principle Name: PIAS2
Official Symbol: PIAS2
All Name and Symbols: PIAS2; protein inhibitor of activated STAT, 2 ; MGC102682; MIZ1; PIASX-ALPHA; PIASX-BETA; SIZ2; miz ; Msx-interacting-zinc finger; protein inhibitor of activated STAT X; ARIP3
Accession Number(s): NP_775298.1; NP_004662.2
Peptide Sequence: KEAMKVSSQPCTKIE, is from internal region
Human Gene ID(s): 9063
Non-Human GeneID(s): 17344 (mouse);
Important Comments: This antibody is expected to recognise both reported isoforms (NP_775298.1 and NP_004662.2). No cross-reactivity expected with PIAS1, 3 and 4.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Preliminary experiments gave an approx 90kDa band in lysates of cell line A431after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of
Immunohistochemistry: In paraffin embedded Human Testis shows nuclear and extra-nuclear staining in spermatids. Recommended concentration, 3-5ug/ml.
Reference(s): van den Akker E, Ano S, Shih HM, Wang LC, Pironin M, Palvimo JJ, Kotaja N, Kirsh O, Dejean A, Ghysdael J. FLI-1 functionally interacts with PIASxalpha, a member of the PIAS E3 SUMO ligase family. J Biol Chem. 2005 Nov 11; 280(45):38035-46. Epub 2005 Sep 7. .PMID: 16148010