Goat anti-Uncoupling protein 2 / UCP2 Antibody

Goat anti-Uncoupling protein 2 / UCP2 Antibody
Principle Name: Uncoupling protein 2 / UCP2
Official Symbol: UCP2
All Name and Symbols: UCP2; uncoupling protein 2 (mitochondrial, proton carrier); SLC25A8; UCPH ; uncoupling protein 2; uncoupling protein-2
Accession Number(s): NP_003346.2
Peptide Sequence: DSVKQFYTKGSEH, is from internal region
Human Gene ID(s): 7351
Non-Human GeneID(s): 22228 (mouse); 54315 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WBPeptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 37kDa band observed in Rat Adipose and 28-30kDa in Mouse Spleen lysates (calculated MW of 33.4kDa according to Mouse NP_035801.3 and 33.4kDa according to Rat NP_062227.2). Recommended concentration: 0.3-1ug/ml. Primary incubation was
Reference(s): De Souza CT, Araujo EP, Stoppiglia LF, Pauli JR, Ropelle E, Rocco SA, Marin RM, Franchini KG, Carvalheira JB, Saad MJ, Boschero AC, Carneiro EM, Velloso LA. Inhibition of UCP2 expression reverses diet-induced diabetes mellitus by effects on both insulin secretion and action. FASEB J. 2007 Jan 5; [Epub ahead of print] .PMID: 17209127