Goat anti-Arginase, type 1 / ARG1 Antibody

Pep ELISA, WB

Goat anti-Arginase, type 1 / ARG1 Antibody
Principle Name: Arginase, type 1 / ARG1
Official Symbol: ARG1
All Name and Symbols: ARG1; arginase, type I; arginase, liver; A-I;
Accession Number(s): NP_000036.2
Peptide Sequence: REGNHKPIDYLNPPK, is from C Terminus
Human Gene ID(s): 383
Important Comments: Please note that ARG1 is not expressed in Human M2 macrophages, but it is in other species (eg rodents).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 2000.
Western Blot: Approx 37kDa band observed in Human Liver lysates (calculated MW of 34.7kDa according to NP_000036.2. Recommended concentration: 0.01-0.03ug/ml. An anonymous customer found positive results in WB on Mouse leukaemic monocyte macrophage cell
Reference(s): Jiang M, Ding Y, Su Y, Hu X, Li J, Zhang Z. Arginase-flotillin interaction brings arginase to red blood cell membrane. FEBS Lett. 2006 Dec 11; 580(28-29):6561-4. Epub 2006 Nov 13. .PMID: 17113085