Goat anti-PSME1 (isoform 1) Antibody

Goat anti-PSME1 (isoform 1) Antibody
Principle Name: PSME1 (isoform 1)
Official Symbol: PSME1
All Name and Symbols: PSME1; proteasome (prosome, macropain) activator subunit 1 (PA28 alpha); IFI5111; MGC8628; PA28A; PA28alpha; REGalpha ; 11S regulator complex alpha subunit; 29-kD MCP activator subunit; activator of multicatalytic protease subunit 1; interferon gamma up-r
Accession Number(s): NP_006254.1
Peptide Sequence: KNFEKLKKPRGETK, is from C Terminus
Human Gene ID(s): 5720
Non-Human GeneID(s): 19186 (mouse); 29630 (rat);
Important Comments: This antibody is expected to recognise isoform 1 (NP_006254.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Approx 30kDa band observed in Human Lymph Node, Spleen, Tonsil and Peripheral Blood Mononuceocyte lysates (calculated MW of 28.7kDa according to NP_006254.1). Recommended concentration: 0.3-1ug/ml.
Reference(s): Brychcy M, Kuckelkorn U, Hausdorf G, Egerer K, Kloetzel PM, Burmester GR, Feist E. Anti-20S proteasome autoantibodies inhibit proteasome stimulation by proteasome activator PA28. Arthritis Rheum. 2006 Jul; 54(7):2175-83..PMID: 16802355