Goat anti-CCKA Receptor (mouse) Antibody

Goat anti-CCKA Receptor (mouse) Antibody
Principle Name: CCKA Receptor (mouse)
Official Symbol: Cckar
All Name and Symbols: Cckar; cholecystokinin A receptor ; AW106902
Accession Number(s): NP_033957.1
Peptide Sequence: KFDASQKKSAKEKR, is from internal region
Human Gene ID(s):
Non-Human GeneID(s): 12425 (mouse); 24889 (rat);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Rat
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Approx. 75kDa band observed in purified membranes of Rat insulinoma cell line RIN14B (calculated MW of 48.4kDa according to NP_033957.1). Data were published by El-Kouhen K and Morisset J. Int J Endocrinol. 2009; 2009:875167; PMID: 20011057.
Reference(s): Bi S, Scott KA, Kopin AS, Moran TH. Differential roles for cholecystokinin a receptors in energy balance in rats and mice. Endocrinology. 2004 Aug; 145(8):3873-80. Epub 2004 May 3. .PMID: 15123537