Description |
Goat anti-HCAP-H Antibody Principle Name: HCAP-H Official Symbol: NCAPH All Name and Symbols: HCAP-H; NCAPH; non-SMC condensin I complex, subunit H; BRRN1; CAP-H; XCAP-H homolog; barren homolog 1; chromosome-associated protein H; condensin complex subunit 2 Accession Number(s): NP_056156.2 Peptide Sequence: TEDLSDVLVRQGD, is from C Terminus Human Gene ID(s): 23397 Non-Human GeneID(s): Important Comments: Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA, WB Tested Species: Human Peptide ELISA: antibody detection limit dilution 1 to 128000. Western Blot: Approx 110kDa band observed in lysates of cell line K562 (calculated MW of 82.6kDa according to NP_056156.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cabello et al, Mol Biol Ce Reference(s): Torres-Rosell J, De Piccoli G, Cordon-Preciado V, Farmer S, Jarmuz A, Machin F, Pasero P, Lisby M, Haber JE, Aragon L. Anaphase onset before complete DNA replication with intact checkpoint responses. Science. 2007 Mar 9; 315(5817):1411-5. .PMID: 17347440 |