Goat anti-RELMbeta Antibody

Pep ELISA, IHC

Goat anti-RELMbeta Antibody
Principle Name: RELMbeta
Official Symbol: RETNLB
All Name and Symbols: RETNLB ; resistin like beta ; FIZZ1; FIZZ2; HXCP2; RELM-beta; RELMb; RELMbeta; XCP2; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 2; colon and small intestine-specific cysteine-rich protein; cysteine-rich secreted A12
Accession Number(s): NP_115968.1
Peptide Sequence: DSVMDKKIKDVLNS, is from internal region
Human Gene ID(s): 84666
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Preliminary experiments gave an approx 35kDa band in Human Ileum and Duodenum lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated
Immunohistochemistry: In paraffin embedded Human Colon shows textured staining of cytoplasm in enterocytes. Recommended concentration, 2-4ug/ml.
Reference(s): McVay LD, Keilbaugh SA, Wong TM, Kierstein S, Shin ME, Lehrke M, Lefterova MI, Shifflett DE, Barnes SL, Cominelli F, Cohn SM, Hecht G, Lazar MA, Haczku A, Wu GD. Absence of bacterially induced RELMbeta reduces injury in the dextran sodium sulfate model of colitis. J Clin Invest. 2006 Nov; 116(11):2914-23. Epub 2006 Oct 5.PMID: 17024245