Goat anti-TRPC4 Antibody

Pep ELISA, WB, IHC

Goat anti-TRPC4 Antibody
Principle Name: TRPC4
Official Symbol: TRPC4
All Name and Symbols: TRPC4; transient receptor potential cation channel, subfamily C, member 4; HTRP4; MGC119570; MGC119571; MGC119572; MGC119573; TRP4; transient receptor potential 4
Accession Number(s): NP_057263.1; NP_003297.1; NP_001129427.1; NP_001129429.1; NP_001129428.1; NP_001129430.1
Peptide Sequence: KHAKEEDSSIDYD, is from internal region (near C Terminus)
Human Gene ID(s): 7223
Non-Human GeneID(s): 22066 (mouse); 84494 (rat);
Important Comments: This antibody is expected to recognise all six reported isoforms (NP_057263.1; NP_003297.1; NP_001129427.1; NP_001129429.1; NP_001129428.1; NP_001129430.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx. 110kDa band observed in Rat Brain lysates (calculated MW of 112kDa according to Human NP_057263.1 and to Rat NP_536321.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.
Immunohistochemistry: Paraffin embedded Human Placenta and Kidney. Recommended concentration: 4.75ug/ml.
Reference(s): Song X, Zhao Y, Narcisse L, Duffy H, Kress Y, Lee S, Brosnan CF. Canonical transient receptor potential channel 4 (TRPC4) co-localizes with the scaffolding protein ZO-1 in human fetal astrocytes in culture. Glia. 2005 Feb; 49(3):418-29.PMID: 15540229