Goat anti-EBF1 / COE1 Antibody

Goat anti-EBF1 / COE1 Antibody
Principle Name: EBF1 / COE1
Official Symbol: EBF1
All Name and Symbols: EBF1; early B-cell factor 1 ; COE1; EBF; O/E-1; OLF1 ; Collier, Olf and EBF transcription factor 1; early B-cell factor olfactory neuronal transcription factor 1
Accession Number(s): NP_076870.1
Peptide Sequence: HNQLPALANTSV, is from internal region
Human Gene ID(s): 1879
Non-Human GeneID(s): 13591 (mouse); 116543 (rat);
Important Comments: This antibody is expected not to cross-react with EBF2, EBF3 and EBF4.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IP
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Approx. 75kDa band observed in Human Adipose lysates (calculated MW of 64.5kDa according to NP_076870.1). Recommended concentration: 0.3-0.1ug/ml.
Reference(s): Lagergren A, Mansson R, Zetterblad J, Smith E, Basta B, Bryder D, Akerblad P, Sigvardsson M. The CXCL12, periostin and CCL9 genes are direct targets for early B-cell factor (EBF) in OP-9 stroma cells. J Biol Chem. 2007 Mar 20; [Epub ahead of print].PMID: 17374609