Goat anti-Desmin Antibody

Pep ELISA, WB

Goat anti-Desmin Antibody
Principle Name: Desmin
Official Symbol: DES
All Name and Symbols: DES; desmin; CMD1I; CSM1; CSM2; FLJ12025; FLJ39719; FLJ41013; FLJ41793; intermediate filament protein
Accession Number(s): NP_001918.3
Peptide Sequence: RDGEVVSEATQQQHE, is from C Terminus
Human Gene ID(s): 1674
Non-Human GeneID(s): 13346 (mouse); 64362 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.Peptide ELISA: antibody detection limit dilution 1 to 4000.
Western Blot: Approx 55-60kDa band observed in Human, Mouse, Rat and Pig Skeletal Muscle lysates (calculated MW of 53.5kDa according to Human NP_001918.3, Mouse NP_034173.1, Rat NP_071976.1 and 53.6kDa according to Pig NP_001001535.1). Recommended concen
Reference(s): Chen F, Chang R, Trivedi M, Capetanaki Y, Cryns VL. Caspase proteolysis of desmin produces a dominant-negative inhibitor of intermediate filaments and promotes apoptosis. J Biol Chem. 2003 Feb 28; 278(9):6848-53.PMID: 12477713