Goat anti-Synaptotagmin I Antibody

Pep ELISA, WB, IHC

Goat anti-Synaptotagmin I Antibody
Principle Name: Synaptotagmin I
Official Symbol: SYT1
All Name and Symbols: synaptotagmin I ; DKFZp781D2042; SYT1; P65; SVP65; SYT; synaptotagmin 1
Accession Number(s): NP_005630.1
Peptide Sequence: RDLQSAEKEEQEK, is from internal region
Human Gene ID(s): 6857
Non-Human GeneID(s): 20979 (mouse); 25716 (rat);
Important Comments: Reported isoforms NP_005630.1, NP_001129277.1 and NP_001129278.1 represent identical protein.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 2000.
Western Blot: Approx. 65kDa band observed in Human Brain (hippocampus) and Mouse and Rat Brain lysates (calculated MW of 47.6kDa according to NP_005630.1). The observed molecular weight corresponds to earlier findings in literature with different antibod
Immunohistochemistry: In paraffin embedded Human Brain shows vesicular staining in neuronal cell and axon. Recommended concentration: 4-6ug/ml.
Reference(s): Martens S, Kozlov MM, McMahon HT. How synaptotagmin promotes membrane fusion. Science. 2007 May 25; 316(5828):1205-8. Epub 2007 May 3.PMID: 17478680