Goat anti-Restin / CLIP1 Antibody

Pep ELISA, WB, IHC

Goat anti-Restin / CLIP1 Antibody
Principle Name: Restin / CLIP1
Official Symbol: CLIP1
All Name and Symbols: CLIP1; CAP-GLY domain containing linker protein 1; CLIP; CLIP-170; CLIP170; CYLN1; MGC131604; RSN; Reed-Steinberg cell-expressed intermediate filament-associated protein; cytoplasmic linker 1; restin; restin (Reed-Steinberg cell-expressed intermediate fil
Accession Number(s): NP_002947.1; NP_937883.1
Peptide Sequence: PHSTHHGSRGEERP, is from C Terminus
Human Gene ID(s): 6249
Non-Human GeneID(s): 56430 (mouse); 65201 (rat);
Important Comments: This antibody is expected to recognize both reported isoforms (NP_002947.1; NP_937883.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Approx 160kDa band observed in Human Breast Cancer lysates (calculated MW of 161kDa according to NP_002947.1. Recommended concentration: 1-3ug/ml.
Immunohistochemistry: Immunohistochemistry: In paraffin embedded Human Thyroid Gland shows structured cytoplasm staining in active epithelial cells. Recommended concentration, 2-4ug/ml.
Reference(s): Tanenbaum ME, Macurek L, Galjart N, Medema RH. Dynein, Lis1 and CLIP-170Counteract Eg5-dependent centrosome separation during bipolar spindle assembly. The EMBO journal 2008 Dec 27 (24): 3235-45.PMID: 19020519