Goat anti-P2RX7 / P2X7 receptor Antibody

Pep ELISA, WB, IHC

Goat anti-P2RX7 / P2X7 receptor Antibody
Principle Name: P2RX7 / P2X7 receptor
Official Symbol: P2RX7
All Name and Symbols: P2RX7; purinergic receptor P2X, ligand-gated ion channel, 7; MGC20089; P2X7; ATP receptor; P2X purinoceptor 7; P2X7 receptor; P2Z receptor; purinergic receptor P2X7
Accession Number(s): NP_002553.2
Peptide Sequence: YETNKVTRIQSMNY, is from N Terminus
Human Gene ID(s): 5027
Non-Human GeneID(s): 18439 (mouse); 29665 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 37kDa and 70kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 68.6kDa according to NP_002553.2). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was
Immunohistochemistry: In paraffin embedded Human Brain Cortex shows membranous staining of cell bodies and processes. Recommended concentration: 3-6ug/ml. Paraffin embedded Human Kidney. Recommended concentration: 3.75ug/ml.
Reference(s): Tomasinsig L, Pizzirani C, Skerlavaj B, Pellegatti P, Gulinelli S, Tossi A, Virgilio FD, Zanetti M, The human cathelicidin LL-37 modulates the activities of the P2X7 receptor in a structure-dependent manner. The Journal of biological chemistry 2008 Nov 283 (45): 30471-81.PMID: 18765670