Description |
Goat anti-IKAROS / IKZF1 Antibody Principle Name: IKAROS / IKZF1 Official Symbol: IKZF1 All Name and Symbols: IKZF1; IKAROS family zinc finger 1 (Ikaros); Hs.54452; IK1; IKAROS; LYF1; PRO0758; ZNFN1A1; hIk-1; CLL-associated antigen KW-6; Ikaros (zinc finger protein); zinc finger protein, subfamily 1A, 1 (Ikaros); lymphoid transcription factor LyF-1 Accession Number(s): NP_006051.1 Peptide Sequence: DTPYDSSASYEKEN, is from internal region Human Gene ID(s): 10320 Non-Human GeneID(s): Important Comments: Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA, WB, IHC Tested Species: Human Peptide ELISA: antibody detection limit dilution 1 to 1000. Western Blot: Approx 65kDa band observed in lysates of cell line MOLT4 (calculated MW of 57.5kDa according to NP_006051.1). Recommended concentration: 1-3ug/ml. Similar results were obtained with EB07847, which was developed against a different part of t Immunohistochemistry: In paraffin embedded Human Tonsil shows textured staining in some nuclei of the lymphoid tissue. Recommended concentration, 2.5-3.8ug/ml. Reference(s): Loeper S, Asa SL, Ezzat S, Ikaros modulates cholesterol uptake: a link between tumor suppression and differentiation. Cancer research 2008 May 68 (10): 3715-23..PMID: 18483254 |