Goat anti-PLA2R1 Antibody

Pep ELISA, WB

Goat anti-PLA2R1 Antibody
Principle Name: PLA2R1
Official Symbol: PLA2R1
All Name and Symbols: PLA2R1; phospholipase A2 receptor 1, 180kDa; CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R; phospholipase A2 receptor 1
Accession Number(s): NP_031392.3; NP_001007268.1; NP_001182570.1
Peptide Sequence: EKKKDTPKQHGT, is from Internal region
Human Gene ID(s): 22925
Important Comments: This antibody is expected to recognize all reported isoforms (NP_031392.3; NP_001007268.1; NP_001182570.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.Peptide ELISA: antibody detection limit dilution 1 to 1000.
Western Blot: Approx 150kDa band observed in Human Kidney and Pig Lung lysates (calculated MW of 153kDa according to NP_001007268.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.
Reference(s): Silliman CC, Moore EE, Zallen G, Gonzalez R, Johnson JL, Elzi DJ, Meng X, Hanasaki K, Ishizaki J, Arita H, Ao L, England KM, Banerjee A. Presence of the M-type sPLA(2) receptor on neutrophils and its role in elastase release and adhesion. American journal of physiology. Cell physiology 2002 Oct 283 (4): C1102-13.PMID: 12225974