Goat anti-NAIP Antibody

Goat anti-NAIP Antibody
Principle Name: NAIP
Official Symbol: NAIP
All Name and Symbols: NAIP; NLR family, apoptosis inhibitory protein; BIRC1; FLJ18088; FLJ42520; FLJ58811; NLRB1; psiNAIP; NLR family, BIR domain containing 1; OTTHUMP00000125255; baculoviral IAP repeat-containing 1; neuronal apoptosis inhibitory protein; nucleotide-binding ol
Accession Number(s): NP_004527.2
Peptide Sequence: QKASDERISQFDHN, is from N Terminus
Human Gene ID(s): 4671
Important Comments: This antibody is expected to recognize reported isoform 1 (NP_004527.2).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Peptide ELISA: antibody detection limit dilution 1 to 1000.
Western Blot: Preliminary experiments in lysates of cell lines A549 and HeLa and in lysates of Human Breast cancer gave no specific signal but low background (at antibody concentration up to 2ug/ml). We would appreciate any feedback from people in the fi
Reference(s): Vinzing M, Eitel J, Lippmann J, Hocke AC, Zahlten J, Slevogt H, N'guessan PD, Günther S, Schmeck B, Hippenstiel S, Flieger A, Suttorp N, Opitz B. NAIP and Ipaf control Legionella pneumophila replication in human cells. J Immunol. 2008 May 15; 180(10):6808-15.PMID: 18453601