Goat anti-TRIM71 (aa390-402) Antibody

Pep ELISA, WB, IHC

Goat anti-TRIM71 (aa390-402) Antibody
Principle Name: TRIM71 (aa390-402)
Official Symbol: TRIM71
All Name and Symbols: TRIM71; tripartite motif-containing 71; LIN-41; LIN41; abnormal cell LINeage LIN-41; homolog of C. elegans Lin-41
Accession Number(s): NP_001034200.1
Peptide Sequence: RQVKAKSLYLQVE, is from internal region
Human Gene ID(s): 131405
Non-Human GeneID(s): 636331 (mouse); 301042 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Approx 100kDa band observed in lysates of the cell lines HEK293 and NIH3T3 (calculated MW of 93.4kDa according to Human NP_001034200.1 and 92.1kDa according to Mouse NP_001035968.1). The observed molecular weight corresponds to findings w
Immunohistochemistry: Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 3.75ug/ml.
Reference(s): Lin YC, Hsieh LC, Kuo MW, Yu J, Kuo HH, Lo WL, Lin RJ, Yu AL, Li WH, Human TRIM71 and its nematode homologue are targets of let-7 microRNA and its zebrafish orthologue is essential for development. Molecular biology and evolution 2007 Nov 24 (11): 2525-34.PMID: 17890240