Goat anti-POU3F3 / BRN1 / OCT8 Antibody

Goat anti-POU3F3 / BRN1 / OCT8 Antibody
Principle Name: POU3F3 / BRN1 / OCT8
Official Symbol: POU3F3
All Name and Symbols: POU3F3; POU class 3 homeobox 3; BRN1; OTF8; OTF-8; POU domain, class 3, transcription factor 3; brain-1; brain-specific homeobox/POU domain protein 1; brn-1; oct-8; octamer-binding protein 8; octamer-binding transcription factor 8
Accession Number(s): NP_006227.1
Peptide Sequence: HMLSHAHQWVTAL, is from internal region
Human Gene ID(s): 5455
Non-Human GeneID(s): 18993 (mouse); 192109 (rat);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Mouse, Rat
Peptide ELISA: antibody detection limit dilution 1 to 4000.
Western Blot: Approx 48kDa band observed in Mouse and Rat Spinal Cord lysates (calculated MW of 50.3kDa according to Human NP_006227.1 and 50.2kDa according to Mouse NP_032926.2). Recommended concentration: 1-3ug/ml.
Reference(s): Castro DS, Skowronska-Krawczyk D, Armant O, Donaldson IJ, Parras C, Hunt C, Critchley JA, Nguyen L, Gossler A, Göttgens B, Matter JM, Guillemot F, Proneural bHLH and Brn proteins coregulate a neurogenic program through cooperative binding to a conserved DNA motif. Developmental cell 2006 Dec 11 (6): 831-44..PMID: 17141158