Goat anti-ACPP / PAP Antibody

Pep ELISA, WB, IHC

Goat anti-ACPP / PAP Antibody
Principle Name: ACPP / PAP
Official Symbol: ACPP
All Name and Symbols: ACPP; acid phosphatase, prostate; ACP-3; ACP3; PAP; prostatic acid phosphotase
Accession Number(s): NP_001090.2
Peptide Sequence: YGIHKQKEKSRLQ, is from internal region
Human Gene ID(s): 55
Non-Human GeneID(s): 56318 (mouse); 56780 (rat);
Important Comments: This antibody is expected to recognize both isoforms (NP_001090.2; NP_001127666.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Approx 50kDa band observed in Human Prostate lysates (calculated MW of 44.6kDa according to NP_001090.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Patel et al, , Clin Chem. 1986 O
Immunohistochemistry: In paraffin embedded Human Prostate shows cytoplasm staining in some secretory cells of the gland. Recommended concentration, 3-5ug/ml.
Reference(s): Quintero IB, Araujo CL, Pulkka AE, Wirkkala RS, Herrala AM, Eskelinen EL, Jokitalo E, Hellström PA, Tuominen HJ, Hirvikoski PP, Vihko PT. Prostatic acid phosphatase is not a prostate specific target. Cancer research 2007 Jul 67 (14): 6549-54.PMID: 17638863