Goat anti-PLA2G4A Antibody

Pep ELISA, WB

Goat anti-PLA2G4A Antibody
Principle Name: PLA2G4A
Official Symbol: PLA2G4A
All Name and Symbols: PLA2G4A; phospholipase A2, group IVA (cytosolic, calcium-dependent); MGC126350; PLA2G4; cPLA2-alpha; calcium-dependent phospholipid-binding protein; cytosolic phospholipase A2, group IVA; lysophospholipase; phosphatidylcholine 2-acylhydrolase
Accession Number(s): NP_077734.1
Peptide Sequence: QEKTFRQQRKEHIR, is from internal region
Human Gene ID(s): 5321
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 90kDa band observed in lysates of cell lines HeLa and NIH3T3 (calculated MW of 85.2kDa according to NP_077734.1). Recommended concentration: 1-3ug/ml.
Reference(s): Gubern A, Barcelo-Torns M, Barneda D, Lopez JM, Masgrau R, Picatoste F, Chalfant CE, Balsinde J, Balboa MA, Claro E, JNK and ceramide kinase govern the biogenesis of lipid droplets through activation of group IVA phospholipase A2. The Journal of biological chemistry 2009 Nov 284 (47): 32359-69.PMID: 19778898