Goat anti-Doublecortin / DCX (aa232-242) Antibody

Goat anti-Doublecortin / DCX (aa232-242) Antibody
Principle Name: Doublecortin / DCX (aa232-242)
Official Symbol: DCX
All Name and Symbols: DCX; doublecortin; RP5-914P14.1; DBCN; DC; FLJ51296; LISX; SCLH; XLIS; OTTHUMP00000023860; OTTHUMP00000216316; doublecortex; doublin; lis-X; lissencephalin-X; neuronal migration protein doublecortin
Accession Number(s): NP_000546.2; NP_835365.1; NP_835364.1; NP_001182482.1
Peptide Sequence: KTSANMKAPQS, is from internal region
Human Gene ID(s): 1641
Non-Human GeneID(s): 13193 (mouse); 84394 (rat);
Important Comments: This antibody is expected to recognize all four reported isoforms (NP_000546.2; NP_835365.1; NP_835364.1; NP_001182482.1). Reported variants represent identical protein: NP_835364.1 and NP_835366.1.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC, IF
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 45kDa band observed in Mouse fetal Brain lysates (calculated MW of 49.3kDa according to Human NP_000546.2 and 40.6kDa according to Human NP_835365.1 and Mouse NP_001103692.1). Recommended concentration: 0.01-0.03ug/ml. Primary incub
Immunohistochemistry: In paraffin embedded Human Cerebellum shows axonal and incidental nuclear staining. Recommended concentration: 2-4ug/ml.
Reference(s): Evangelisti C, Florian MC, Massimi I, Dominici C, Giannini G, Galardi S, Buè MC, Massalini S, McDowell HP, Messi E, Gulino A, Farace MG, Ciafrè SA, MiR-128 up-regulation inhibits Reelin and DCX expression and reduces neuroblastoma cell motility and invasiveness. The FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2009 Dec 23 (12): 4276-87.PMID: 19713529