Description |
Goat anti-Scn10a / Nav1.8 (mouse) Antibody Principle Name: Scn10a / Nav1.8 (mouse) Official Symbol: Scn10a All Name and Symbols: Scn10a; sodium channel, voltage-gated, type X, alpha subunit; Nav1.8; PN3; SNS; mPN3; peripheral nerve sodium channel 3; sodium channel protein type 10 subunit alpha; sodium channel protein type X subunit alpha; sodium channel, voltage-gated, type X, alph Accession Number(s): NP_033160.2 Peptide Sequence: DDNRSLQSDPYNQR, is from internal region Human Gene ID(s): 6336 Non-Human GeneID(s): 20264 (mouse); 29571 (rat); Important Comments: Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA Tested Species: Peptide ELISA: antibody detection limit dilution 1 to 32000. Western Blot: Preliminary experiments gave an approx 150kDa band in Rat Spinal Cord and NIH3T3 lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated Reference(s): Li Q, Su YY, Wang H, Li L, Wang Q, Bao L, Transmembrane segments prevent surface expression of sodium channel Nav1.8 and promote calnexin-dependent channel degradation. The Journal of biological chemistry 2010 Oct 285 (43): 32977-87..PMID: 20720009 |