Goat anti-IDH1 (yeast) Antibody

Pep ELISA, WB

Goat anti-IDH1 (yeast) Antibody
Principle Name: IDH1 (yeast)
Official Symbol: IDH1
All Name and Symbols: IDH1; Idh1p; YNL037C; Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle
Accession Number(s): NP_014361.1
Peptide Sequence: EPGSRHVGLDIKGQN, is from internal region
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 38kDa band observed in wildtype lysates of S.cerevisiae (calculated MW of 39.3kDa according to NP_014361.1). Recommended concentration: 0.3-1ug/ml.
Reference(s): Helbig AO, de Groot MJ, van Gestel RA, Mohammed S, de Hulster EA, Luttik MA, Daran-Lapujade P, Pronk JT, Heck AJ, Slijper M. A three-way proteomics strategy allows differential analysis of yeast mitochondrial membrane protein complexes under anaerobic and aerobic conditions. Proteomics. 2009 Oct; 9(20):4787-98.19750512.