Goat anti-Arl6 / BBS3 (mouse) Antibody

Pep ELISA, IHC

Goat anti-Arl6 / BBS3 (mouse) Antibody
Principle Name: Arl6 / BBS3 (mouse)
Official Symbol: Arl6
All Name and Symbols: ARL6; ADP-ribosylation factor-like 6; BBS3; 1110018H24Rik; 2210411E14Rik; ADP-ribosylation factor-like protein 6; ADP-ribosylation-like factor homolog ARL6; bardet-Biedl syndrome 3 protein
Accession Number(s): NP_062639.3
Peptide Sequence: KPSNAQSQDIVPT, is from internal region
Non-Human GeneID(s): 56297 (mouse);
Important Comments: Reported variants represent identical protein: NP_816931.1, NP_115522.1
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Preliminary experiments gave an approx 33kDa band in Mouse Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 21.0kDa
Immunohistochemistry: Paraffin embedded Human Lung. Recommended concentration: 5ug/ml.
Reference(s): Wiens CJ, Tong Y, Esmail MA, Oh E, Gerdes JM, Wang J, Tempel W, Rattner JB, Katsanis N, Park HW, Leroux MR, Bardet-Biedl syndrome-associated small GTPase ARL6 (BBS3) functions at or near the ciliary gate and modulates Wnt signaling. The Journal of biological chemistry 2010 May 285 (21): 16218-30.PMID: 20207729