Goat anti-Hsd11b1 (mouse) Antibody

Pep ELISA, WB

Goat anti-Hsd11b1 (mouse) Antibody
Principle Name: Hsd11b1 (mouse)
Official Symbol: Hsd11b1
All Name and Symbols: Hsd11b1; hydroxysteroid (11-beta) dehydrogenase 1; 11-DH; 11-beta-HSD1; HDL; HSD11; HSD11B; HSD11L; 11-beta-hydroxysteroid dehydrogenase 1; corticosteroid 11-beta-dehydrogenase isozyme 1; short chain dehydrogenase/reductase family 26C, member 1
Accession Number(s): NP_032314.2
Peptide Sequence: TARSEEGLQKVVSR, is from internal region
Non-Human GeneID(s): 15483 (mouse); 25116 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 32kDa band observed in Mouse Lung lysates (calculated MW of 32.4kDa according to NP_032314.2). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 45kDa. This band was succe
Reference(s): Sinclair AJ, Walker EA, Burdon MA, van Beek AP, Kema IP, Hughes BA, Murray PI, Nightingale PG, Stewart PM, Rauz S, Tomlinson JW. Cerebrospinal fluid corticosteroid levels and cortisol metabolism in patients with idiopathic intracranial hypertension: a link between 11beta-HSD1 and intracranial pressure regulation? J Clin Endocrinol Metab. 2010 Dec; 95(12):5348-56.PMID: 20826586