Goat anti-DBH (aa437-448) Antibody

Pep ELISA, WB, IHC

Goat anti-DBH (aa437-448) Antibody
Principle Name: DBH (aa437-448)
Official Symbol: DBH
All Name and Symbols: DBH; dopamine beta-hydroxylase (dopamine beta-monooxygenase); DBM; dopamine beta-hydroxylase
Accession Number(s): NP_000778.3
Peptide Sequence: DNHYSPHFQEIR, is from internal region
Human Gene ID(s): 1621
Non-Human GeneID(s): 13166 (mouse); 25699 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 70kDa band observed in lysates of cell line Jurkat (calculated MW of 69.1kDa according to NP_000778.3). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat Brain f
Immunohistochemistry: Paraffin embedded Human Adrenal Gland. Recommended concentration: 5ug/ml.
Reference(s): Kim CH, Leung A, Huh YH, Yang E, Kim DJ, Leblanc P, Ryu H, Kim K, Kim DW, Garland EM, Raj SR, Biaggioni I, Robertson D, Kim KS. Norepinephrine deficiency is caused by combined abnormal mRNA processing and defective protein trafficking of dopamine beta-hydroxylase. J Biol Chem. 2011 Mar 18; 286(11):9196-204. Epub 2011 Jan 5.PMID: 21209083