Goat anti-PDE4B Antibody

Pep ELISA, WB

Goat anti-PDE4B Antibody
Principle Name: PDE4B
Official Symbol: PDE4B
All Name and Symbols: PDE4B; phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila); DPDE4; PDEIVB; Phosphodiesterase-4B, cAMP-specific (dunce (Drosophila)-homolog; phosphodiesterase 4B, cAMP-specific (dunce (Drosophila)-homolog phosphodiesterase
Accession Number(s): NP_002591.2; NP_001032416.1; NP_001032417.1
Peptide Sequence: IDIATEDKSPVDT, is from C Terminus
Human Gene ID(s): 5142
Non-Human GeneID(s): 18578 (mouse);
Important Comments: This antibody is expected to recognize all reported isoforms (NP_002591.2; NP_001032416.1; NP_001032417.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 98kDa band observed in Human Uterus lysates (calculated MW of 83.3kDa according to NP_002591.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Mehats et al, Endocrinology. 1999