Goat anti-RIF1 Antibody

Pep ELISA, WB

Goat anti-RIF1 Antibody
Principle Name: RIF1
Official Symbol: RIF1
All Name and Symbols: RIF1; RAP1 interacting factor homolog (yeast); DKFZp434D1026; DKFZp781N1478; FLJ12870; OTTHUMP00000162712; OTTHUMP00000207206; OTTHUMP00000207207; OTTHUMP00000207208; OTTHUMP00000207210; rap1-interacting factor 1 homolog; telomere-associated protein RIF1
Accession Number(s): NP_060621.3; NP_001171134.1
Peptide Sequence: RNLQSRWRSPSHEN, is from C Terminus
Human Gene ID(s): 55183
Non-Human GeneID(s): 51869 (mouse);
Important Comments: This antibody is expected to recognize both reported isoforms (NP_060621.3; NP_001171134.1). The immunizing peptide sequence has been optimised for cross-reactiviy in mouse. Reported variants represent identical protein: NP_001171135.1, NP_001171136.1, NP
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Approx 270kDa band observed in lysates of cell line HeLa and of Mouse Embryonic Stem cells (calculated MW of 274kDa according to NP_060621.3). The observed band is suppressed by introduction of Rif1 siRNA to HeLa. Recommended concentration:
Reference(s): Wang H, Zhao A, Chen L, Zhong X, Liao J, Gao M, Cai M, Lee DH, Li J, Chowdhury D, Yang YG, Pfeifer GP, Yen Y, Xu X. Human RIF1 encodes an anti-apoptotic factor required for DNA repair. Carcinogenesis. 2009 Aug; 30(8):1314-9.PMID: 19483192