Goat anti-complement C3 (aa1580-1592) Antibody

Goat anti-complement C3 (aa1580-1592) Antibody
Principle Name: complement C3 (aa1580-1592)
Official Symbol: C3
All Name and Symbols: C3; complement component 3; AHUS5; ARMD9; ASP; CPAMD1; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; OTTHUMP00000197086; acylation-stimulating protein cleavage product; complement C3; complement component C3
Accession Number(s): NP_000055.2
Peptide Sequence: QQRTFISPIKCRE, is from C Terminus
Human Gene ID(s): 718
Important Comments: This antibody is expected to recognize the C3 alpha chain, C3b alpha' chain and the C3c alpha' chain fragment 2.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Preliminary experiments gave an approx 125kDa band in Human Liver lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 104k
Reference(s): Kenyon KD, Cole C, Crawford F, Kappler JW, Thurman JM, Bratton DL, Boackle SA, Henson PM. IgG autoantibodies against deposited C3 inhibit macrophage-mediated apoptotic cell engulfment in systemic autoimmunity. J Immunol. 2011 Sep 1; 187(5):2101-11.PMID: 21813769