Goat anti-G-CSF receptor Antibody

Goat anti-G-CSF receptor Antibody
Principle Name: G-CSF receptor
Official Symbol: CSF3R
All Name and Symbols: CSF3R; colony stimulating factor 3 receptor (granulocyte); CD114; GCSFR; CD114 antigen; G-CSF receptor; G-CSF-R; OTTHUMP00000009703; OTTHUMP00000009704; OTTHUMP00000009705; granulocyte colony-stimulating factor receptor
Accession Number(s): NP_000751.1; NP_724781.1; NP_758519.1
Peptide Sequence: TQGDSILDCVPKDGQ, is from internal region
Human Gene ID(s): 1441
Important Comments: This antibody is expected to recognize all reported isoforms (NP_000751.1; NP_724781.1; NP_758519.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Peptide ELISA: antibody detection limit dilution 1 to 4000.
Western Blot: Preliminary experiments in Human Placenta, Bone marrow and peripheral lymphocytes lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have
Reference(s): Meenhuis A, Verwijmeren C, Roovers O, Touw IP. The deubiquitinating enzyme DUB2A enhances CSF3 signalling by attenuating lysosomal routing of the CSF3 receptor. Biochem J. 2011 Mar 1; 434(2):343-51.PMID: 21155715