Goat anti-DUSP6 / MKP3 Antibody

Pep ELISA, WB

Goat anti-DUSP6 / MKP3 Antibody
Principle Name: DUSP6 / MKP3
Official Symbol: DUSP6
All Name and Symbols: DUSP6; dual specificity phosphatase 6; MKP3; PYST1; MAP kinase phosphatase 3; dual specificity protein phosphatase 6; dual specificity protein phosphatase PYST1; mitogen-activated protein kinase phosphatase 3; serine/threonine specific protein phosphatase
Accession Number(s): NP_001937.2; NP_073143.2
Peptide Sequence: PSNQNVYQVDSLQST, is from C Terminus
Human Gene ID(s): 1848
Non-Human GeneID(s): 67603 (mouse); 116663 (rat);
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001937.2; NP_073143.2).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 26kDa band observed in Mouse and Rat Heart lysates and 40kDa plus 26kDa bands observed in Human and Pig Heart lysates (calculated MW of 42.3kDa according to NP_001937.2 and 26.5kDa according to NP_073143.2). Recommended concentration
Reference(s): Geetha N, Mihaly J, Stockenhuber A, Blasi F, Uhrin P, Binder BR, Freissmuth M, Breuss JM. Signal integration and coincidence detection in the mitogen-activated protein kinase/extracellular signal-regulated kinase (ERK) cascade: concomitant activation of receptor tyrosine kinases and of LRP-1 leads to sustained ERK phosphorylation via down-regu J Biol Chem. 2011 Jul 22; 286(29):25663-74.PMID: 21610072