Goat anti-Ghrelin O-acyltransferase Antibody

Goat anti-Ghrelin O-acyltransferase Antibody
Principle Name: Ghrelin O-acyltransferase
Official Symbol: MBOAT4
All Name and Symbols: MBOAT4; membrane bound O-acyltransferase domain containing 4; GOAT; OACT4; O-acyltransferase (membrane bound) domain containing 4; O-acyltransferase domain-containing protein 4; ghrelin O-acyltransferase; membrane-bound O-acyltransferase domain-containing
Accession Number(s): NP_001094386.1
Peptide Sequence: QSPGEEGYVPDAD, is from internal region
Human Gene ID(s): 619373
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Peptide ELISA: antibody detection limit dilution 1 to 2000.
Western Blot: Preliminary experiments in Human Duodenum, Ileum and Pancreas lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results been rep
Reference(s): Takahashi T, Ida T, Sato T, Nakashima Y, Nakamura Y, Tsuji A, Kojima M, Production of n-octanoyl-modified ghrelin in cultured cells requires prohormone processing protease and ghrelin O-acyltransferase, as well as n-octanoic acid. Journal of biochemistry 2009 Nov 146 (5): 675-82.PMID: 19628676