Goat anti-Stomatin Antibody

Pep ELISA, WB, IHC

Goat anti-Stomatin Antibody
Principle Name: Stomatin
Official Symbol: STOM
All Name and Symbols: STOM; stomatin; BND7; EPB7; EPB72; erythrocyte band 7 integral membrane protein; erythrocyte membrane protein band 7.2 (stomatin); protein 7.2b
Accession Number(s): NP_004090.4; NP_937837.1
Peptide Sequence: EKRHTRDSEAQR, is from N Terminus
Human Gene ID(s): 2040
Important Comments: This antibody is expected to recognize both reported isoforms (NP_004090.4; NP_937837.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 30kDa band observed in Human Erythrocytes, Spleen, Bone Marrow and Lung lysates (calculated MW of 31.7kDa according to NP_004090.4). Recommended concentration: 0.1-0.3ug/ml.
Immunohistochemistry: In paraffin embedded Human Liver shows membrane staining of hepatocytes and of sinusoidal lining cells. Recommended concentration, 5-10ug/ml.
Reference(s): Mrowczynska L, Salzer U, Perutkova S, Iglic A, Hägerstrand H. Echinophilic proteins stomatin, sorcin, and synexin locate outside gangliosideM1 (GM1) patches in the erythrocyte membrane. Biochem Biophys Res Commun. 2010 Oct 22; 401(3):396-400.PMID: 20858460