Goat anti-RPA1 / RPA70 (aa323-337) Antibody

Pep ELISA, WB

Goat anti-RPA1 / RPA70 (aa323-337) Antibody
Principle Name: RPA1 / RPA70 (aa323-337)
Official Symbol: RPA1
All Name and Symbols: RPA1; replication protein A1, 70kDa; HSSB; MST075; REPA1; RF-A; RP-A; RPA70; MSTP075; RF-A protein 1; RP-A p70; replication factor A protein 1; replication protein A 70 kDa DNA-binding subunit; single-stranded DNA-binding protein
Accession Number(s): NP_002936.1
Peptide Sequence: CKSYEDATKITVRSN, is from internal region
Human Gene ID(s): 6117
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 70+80kDa band observed in lysates of cell line HeLa (calculated MW of 68.1kDa according to NP_002936.1). The upper band likely represents the acetylated protein. A 75kDa band was observed in several Mouse cell lines and in lysates of
Reference(s): Lada AG, Waisertreiger IS, Grabow CE, Prakash A, Borgstahl GE, Rogozin IB, Pavlov YI. Replication protein A (RPA) hampers the processive action of APOBEC3G cytosine deaminase on single-stranded DNA. PLoS One. 2011; 6(9):e24848.PMID: 21935481