Goat anti-bcl-2-related protein A1 (rat) Antibody

Pep ELISA, WB

Goat anti-bcl-2-related protein A1 (rat) Antibody
Principle Name: bcl-2-related protein A1 (rat)
Official Symbol: Bcl2a1a
All Name and Symbols: Bcl2a1; BCL2-related protein A1; Bcl2a1d; B-cell leukemia/lymphoma 2 related protein A1; B-cell leukemia/lymphoma 2 related protein A1d; BCL2-related protein A1d; bcl-2-related protein A1
Accession Number(s): NP_596907.1
Peptide Sequence: QKEVEKNLKPYLDD, is from internal region
Non-Human GeneID(s): 12044 (mouse); 170929 (rat);
Important Comments: This antibody may cross-react with the almost identical proteins A1b (GeneID 12045) and A1d (GeneID 12047).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 19kDa band observed in Rat Spleen lysates (calculated MW of 20.4kDa according to NP_NP_596907.1). Recommended concentration: 0.1-0.3ug/ml. Antibody tested negaitve in mouse cells.
Reference(s): Cruz-Muñoz W, Jaramillo ML, Man S, Xu P, Banville M, Collins C, Nantel A, Francia G, Morgan SS, Cranmer LD, O'Connor-McCourt MD, Kerbel RS. Roles for endothelin receptor B and BCL2A1 in spontaneous CNS metastasis of melanoma. Cancer research 2012 Oct 72 (19): 4909-19.PMID: 22865454