Goat anti-Il6ra (mouse) Antibody

Goat anti-Il6ra (mouse) Antibody
Principle Name: Il6ra (mouse)
Official Symbol: Il6ra
All Name and Symbols: Il6ra; interleukin 6 receptor, alpha; CD126; IL-6R; Il6r; MGC30256; IL-6 receptor alpha chain; IL-6 receptor subunit alpha; IL-6R 1; IL-6R subunit alpha; IL-6R-alpha; IL-6R
Accession Number(s): NP_034689.2
Peptide Sequence: YLLQFQLRYRPVWSK, is from internal region
Non-Human GeneID(s): 16194 (mouse); 24499 (rat);
Important Comments: This antibody is expected to recognize all reported isoforms (NP_000556.1; NP_852004.1). The sequence of the immunizing peptide corresponds with a part of the extracellular domain.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Peptide ELISA: antibody detection limit dilution 1 to 2000.
Western Blot: Preliminary experiments in Mouse Spleen and Thymus lysates and in lysates of cell lines NIH3T3 and NSO gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the
Reference(s): Boulanger MJ, Chow DC, Brevnova EE, Garcia KC. Hexameric structure and assembly of the interleukin-6/IL-6 alpha-receptor/gp130 complex. Science. 2003 Jun 27; 300(5628):2101-4. Erratum in: Science. 2003 Aug 15; 301(5635):918.PMID: 12829785