Goat anti-SGK Antibody

Pep ELISA, IHC

Goat anti-SGK Antibody
Principle Name: SGK
Official Symbol: SGK1
All Name and Symbols: SGK1; serum/glucocorticoid regulated kinase 1; SGK; OTTHUMP00000017247; serine/threonine protein kinase SGK; serum and glucocorticoid regulated kinase
Accession Number(s): NP_005618.2
Peptide Sequence: TEEPVPNSIGKSPD, is from C Terminus
Human Gene ID(s): 6446
Non-Human GeneID(s): 20393 (mouse); 29517 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 4000.
Immunohistochemistry: In paraffin embedded Human Kidney shows membrane and nuclear stains in epithelial cells of DCT. Recommended concentration, 2-4ug/ml.
Reference(s): Dehner M, Hadjihannas M, Weiske J, Huber O, Behrens J, Wnt signaling inhibits Forkhead box O3a-induced transcription and apoptosis through up-regulation of serum- and glucocorticoid-inducible kinase 1. J Biol Chem. 2008 Jul 11; 283(28):19201-10.PMID: 18487207