Goat anti-carbonic anhydrase XII (aa188-199) Antibody

Pep ELISA, WB, IHC

Goat anti-carbonic anhydrase XII (aa188-199) Antibody
Principle Name: carbonic anhydrase XII (aa188-199)
Official Symbol: CA12
All Name and Symbols: CA12; carbonic anhydrase XII; CAXII; HsT18816; CA-XII; carbonate dehydratase XII; carbonic anhydrase 12; carbonic dehydratase; tumor antigen HOM-RCC-3.1.3
Accession Number(s): NP_001209.1; NP_996808.1; NP_001280571.1
Peptide Sequence: SHLQHVKYKGQE, is from internal region
Human Gene ID(s): 771
Important Comments: This antibody is expected to recognize all reported isoforms (NP_001209.1; NP_996808.1; NP_001280571.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 45kDa band observed in Human Kidney lysates (calculated MW of 39.5kDa according to NP_001209.1). Recommended concentration: 0.3-1ug/ml.
Immunohistochemistry: In paraffin embedded Human Kidney shows membranous staining in some epithelial cells of convoluted tubules. Recommended concentration, 5-10ug/ml.
Reference(s): Muhammad E et al. Autosomal recessive hyponatremia due to isolated salt wasting in sweat associated with a mutation in the active site of Carbonic Anhydrase 12. Hum Genet. 2011 Apr; 129(4):397-405.PMID: 21184099